Journal: Cancers
Article Title: ERK MAP Kinase Signaling Regulates RAR Signaling to Confer Retinoid Resistance on Breast Cancer Cells
doi: 10.3390/cancers14235890
Figure Lengend Snippet: Ligand-dependent induction of RAR-target genes is suppressed by activation of RAS-ERK signaling. ( A ) A HA-tagged dominant negative form of RARα (HA-DN-RARα) was expressed in MCF7 cells by using lentivirus. Cells were treated with DMSO or RA (1 μM) for 24 h prior to the analyses. Total RNA and proteins were analyzed by RT-PCR (left) and immunoblotting (right), respectively. *, p < 0.05 (one-way ANOVA followed by Tukey post hoc analyses) (mean + s.e.m., n = 4). ( B ) KRAS-V12 was expressed in MCF7 cells by using lentivirus. 4 days after infection of lentivirus, cells were treated with DMSO or RA (1 μM) for 24 h. Total RNA and proteins were analyzed by RT-PCR (left) and immunoblotting (right), respectively. *, p < 0.05 (one-way ANOVA followed by Tukey post hoc analyses) (mean + s.e.m., n = 4). ( C ) KRAS-V12 was expressed in MCF10A cells by using lentivirus. 3 days after infection of lentivirus, cells were treated with DMSO or RA (1 μM) for 24 h. The indicated mRNA levels were analyzed by RT-PCR. (left) Morphology of MCF10A cells expressing KRAS-V12 or control cells. Scale bars: 50 μm. *, p < 0.05 (one-way ANOVA followed by Tukey post hoc analyses) (mean + s.e.m., n = 3). Uncropped immunoblot images are shown in .
Article Snippet: All cell lines used in this study (MCF-7, MDA-MB-231, MCF10A, and HEK293T) were obtained from American Type Culture Collection.
Techniques: Activation Assay, Dominant Negative Mutation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Infection, Expressing, Control
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